blot n. 1.墨污,墨渍,污点,污斑。 2.瑕疵;耻辱,污名。 3.〔古语〕涂去,抹去。 a blot on her past [her character] 她历史[品格]上的污点。 drop an ink blot on an envelope 信封玷上了一块墨污。 vt. (-tt-) 1.用(墨水等)弄脏,涂污。 2.涂去,抹掉(out)。 3.用吸墨纸吸干。 4.遮蔽(风景等),遮暗,使昏暗。 5.玷污,损害(名誉等)。 6.〔美俚〕杀死;乱涂,乱写;〔诗〕擦掉。 The sun was blotted by the moon. 月亮遮暗了太阳,日蚀。 blot the wet pane 擦干潮湿的窗玻璃。 blotting pad 吸墨纸滚台。 vi. 1.(墨水)渗开;造成污渍。 2.(纸等)玷上墨污;(吸墨纸)吸掉墨水。 This pen blots. 这枝笔漏墨水。 This paper blots easily. 这种纸容易吸水。 blot one's copybook 损坏自己名誉。 blot out 1. 涂去(文字)。 2. 遮掉(风景等),遮暗。 3. 〔美俚〕消灭,杀掉;毁掉。 n. 1.(十五子棋中)易被吃掉的孤立棋子。 2.弱点。 hit a blot 吃去弱子,突破弱点。
dot n. 多特 〔Dorothea 的昵称〕。 n. 1.点;圆点;句点;【音乐】附点〔音符后的一点,表示延长1/2拍〕。 2.一点点大的东西,小片,少量,小个子。 3.【数学】小数点;相乘的符号。 There are dots of soot on the window sill. 窗台上有一点烟灰。 Put a dot on [over] the i. 给字母 i 加上一点。 a dot of butter 一点儿奶油。 a mere dot of a child 小小的孩子。 in the year dot 〔口语〕老早以前。 off one's dot 〔俚语〕傻头傻脑的;发疯。 on the dot 〔口语〕按时;准时。 put dots on 〔口语〕使倦怠[烦闷]。 to a dot 〔美国〕完全,全部 (be correct to a dot 完全正确)。 to the dot of an i 一丝不苟地。 vt. (-tt-) 1.打点于;星罗棋布于,点缀;用点线表示。 2.〔俚语〕敲,打。 Dot your i's and j's. 写字时不要忘记在i和j字上打一点。 the sea dotted with ships 点缀着船只的海洋。 Trees dot the landscape. 树木点缀着景色。 Stars dot the sky. 星星缀满天空。 a dotted note 【音乐】附点音符。 dot a line across the page 横贯书页划一条虚线。 dot sb. in the eye 一拳打到某人眼上。 a dotted line 虚线。 dot and carry one 〔做加法时〕逢十进位〔儿童用语〕。 dot and go one 1. = dot and carry one. 2.n. (拄着丁字杖走的)瘸子;一瘸一拐的步行声。 3. 一瘸一拐的[地]。 dot down 暂且记下来。 dot one's i's and cross one's t's 打 i 的点画 t 的横线;一笔不苟,详述。 sign on the dotted line 〔美国〕在(信封、文件等留出供署名的)虚线上签名;〔比喻〕全盘接受。 n. 【法律】嫁妆,嫁资,妆奁。 adj. -al
Bl21 . the dot blot hybridization verified that the scfv was secreted to the medium 从培养基中可检测到可溶性抗人绒毛膜促性腺激素的单链抗体。
Lastly by using the technique of dot blot hybridization , the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer . the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe . by comparing the diagnostic ways of nucleotide probe and fc , the technique of nucleotide probe were proved to have high sensitivity and speci fi city 最后,用地高辛随机引物法标记成momp基因核酸探针,斑点杂交检测衣原体基因组dna ,灵敏度可达10pg ,且不能检出其它病原体的核酸。将核酸探针法与补体结合反应法对衣原体感染的诊断进行比较,初步证明该探针具有较高的敏感性与较强的特异性。
The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii . two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium . the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome . at the same time ; compared agrabactenum - mediated method with gene gun method , the transformation frequency of the former was 16 . 7 % , while the latter was 50 % , so gene gun transformation method was suitable for long ya liiliwn 用携带有几丁质酶基因和- 1 、 3葡聚糖酶基因的工程菌,通过农杆菌介导法和基因枪转化法转化龙牙百合,经pcr和点杂交检测证明外源基因已经整合到植物染色体中。同时对农杆菌介导法和基因枪法进行比较,发现农杆菌介导法的转化率为16 . 7 ,基因枪法的转化率为50 ,因此可能基因枪转化法更适于龙牙百合的遗传转化。
Was multiplied and the tk gene was cloned . the cloned tk gene was retrieved by proper restrictive hemodynamics . the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin . then , the specificity and sensitivity of tk gene probe were detected with dot blot hybridization . the sequence of tk gene of nm98a strain was analysed . the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99 . 7 % 本研究中首次对iltv - nm98a株的tk基因进行了克隆和序列分析,结果表明: iltv - nm98a株tk基因的核苷酸序列与已发表的iltvtk基因的核苷酸序列具有高度的同源性,两者之间仅相差4个核苷酸,同源性高达99 . 7 ,从而证实了iltvtk基因是高度保守的,为iltvtk基因核酸探针的制备提供了有力的依据。